Anticancer activity of some polyamine derivatives on human prostate and breast cancer cell lines

Marta Szumilak; Malgorzata Galdyszynska; Kamila Dominska; Andrzej Stanczak; Agnieszka Wanda Piastowska-Ciesielska

doi:10.18388/abp.2016_1416

Marta Szumilak
Malgorzata Galdyszynska
Kamila Dominska
Andrzej Stanczak
Agnieszka Wanda Piastowska-Ciesielska Department of Comparative Endocrinology, Faculty of Biomedical Sciences and Postgraduate Training, Medical University of Lodz,

DOI: https://doi.org/10.18388/abp.2016_1416

Keywords: polyamine derivatives, prostate cancer, breast cancer, mitochondrial potential, cell cycle, apop-tosis

Abstract

The aim of this study was to expand our knowledge about anticancer activity of some polyamine derivatives with quinoline or chromane as terminal moieties. Tested compounds were evaluated in vitro towards metastatic human prostate adenocarcinoma (PC3), human carcinoma (DU145) and mammary gland adenocarcinoma (MCF7) cell lines. Cell viability was estimated on the basis of mitochondrial metabolic activity using watersoluble tetrazolium WST1 to establish effective concentrations of the tested compounds under experimental conditions. Cytotoxic potential of polyamine derivatives was determined by the measurement of lactate dehydrogenase activity released from damaged cells, changes in mitochondrial membrane potential, the cell cycle distribution analysis and apoptosis assay. It was revealed that the tested polyamine derivatives differed markedly in their antiproliferative activity. Bischromane derivative 5a exhibited a rather cytostatic than cytotoxic effect on the tested cells, whereas quinoline derivative 3a caused changes in cell membrane integrity, inhibited cell cycle progression, as well as induced apoptosis of prostate and breast cancer cells which suggest its potential application in cancer therapy.

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