ESR1 and GPX1 genes expression level in human malignant and non-malignant breast tissues

Magdalena Beata Król; Michał Galicki; Peter Grešner; Edyta Wieczorek; Ewa Jabłońska; Edyta Reszka; Zbigniew Morawiec; Wojciech Wąsowicz; Jolanta Gromadzińska

doi:10.18388/abp.2016_1425

Magdalena Beata Król Department of Biological and Environmental Monitoring, Nofer Institute of Occupational Medicine, Łódź, Poland http://orcid.org/0000-0001-7856-2968
Michał Galicki Department of Surgical Oncology, Copernicus Memorial Hospital, Cancer Center, Łódź, Poland
Peter Grešner Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Łódź, Poland
Edyta Wieczorek Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Łódź, Poland
Ewa Jabłońska Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Łódź, Poland
Edyta Reszka Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Łódź, Poland
Zbigniew Morawiec Department of Surgical Oncology, Copernicus Memorial Hospital, Cancer Center, Łódź, Poland
Wojciech Wąsowicz Department of Biological and Environmental Monitoring, Nofer Institute of Occupational Medicine, Łódź, Poland
Jolanta Gromadzińska Department of Biological and Environmental Monitoring, Nofer Institute of Occupational Medicine, Łódź, Poland

DOI: https://doi.org/10.18388/abp.2016_1425

Abstract

Background: The aim of this study was to find out whether the mRNA expression of estrogen receptor alpha (encoded by ESR1) correlates with the expression of glutathione peroxidase 1 (encoded by GPX1) in tumor and adjacent tumor-free breast tissue and whether this correlation is affected by breast cancer. Such relationships may give further insights into breast cancer pathology with respect to the status of estrogen receptor.

Methods: We used the quantitative real-time PCR technique to analyze differences in the expression levels of the ESR1 and GPX1 genes in paired malignant and non-malignant tissues from breast cancer patients.

Results: ESR1 and GPX1 expression levels were found to be significantly down-regulated by 14.7% and 7.4% (respectively) in the tumorous breast tissue compared to the non-malignant one. Down-regulation of these gene were independent of tumor histopathology classification and clinicopathological factors while ESR1 mRNA level was reduced with increasing tumor grade (G1: 103% vs. G2: 85.8% vs. G3: 84.5%; p<0.05). In the non-malignant and malignant breast tissues, the expression levels of ESR1 and GPX1 were significantly correlated with each other (Rs=0.450 and Rs=0.360; respectively).

Conclusion: These data suggest that down-regulation of ESR1 and GPX1 are independent on clinicopathological factors. Down-regulation of ESR1 gene expression enhanced with the development of the disease. Moreover, GPX1 and ESR1 genes expression are interdependent in the malignant breast tissue and further work is needed to determine the mechanism underlying this relationship.

Author Biography

Magdalena Beata Król, Department of Biological and Environmental Monitoring, Nofer Institute of Occupational Medicine, Łódź, Poland

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional research committee (resolution no. 01/2011) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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