Rhizobium strains differ considerably in outer membrane permeability and polymyxin B resistance

Iwona Komaniecka; Katarzyna Zamłyńska; Radosław Zan; Magdalena Staszczak; Jarosław Pawelec; Irena Seta; Adam Choma

doi:10.18388/abp.2015_1212

Rhizobium strains differ considerably in outer membrane permeability and polymyxin B resistance

Iwona Komaniecka
Katarzyna Zamłyńska
Radosław Zan
Magdalena Staszczak
Jarosław Pawelec
Irena Seta
Adam Choma

DOI: https://doi.org/10.18388/abp.2015_1212

Abstract

Six rhizobium (Rhizobium leguminosarum bv. Trifolii TA1, Sinorhizobium meliloti 1021, Mesorhizobium huakuii IFO 15243^T, Ochrobactrum lupini LUP 21^T, Bradyrhizobium japonicum USDA110 and B. elkaniiUSDA 76) and two Escherichia coli strains (E. coli ATCC 25922 and E. coli HB 101) were compared in respect to polymyxin B and EDTA resistance, as well as bacterial outer membrane (OM) permeability to a fluorescent hydrophobic agent (N-phenyl-1-naphthylamine - NPN). TEM (Transmission Electron Microscopy) and a microbial test demonstrated that all the rhizobia were much more resistant to polymyxin B in comparison with E. coli strains. EDTA and polymyxin B enhance permeability of B. japonicum and O. lupini OM. Other rhizobia incorporated NPN independently of the presence of membrane-deteriorating agents; however, the level of fluorescence (measured as NPN absorption) was strain dependent.

Published

2016-06-02

Issue

Vol. 63 No. 3 (2016)

Section

Articles

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