Oxalic acid degradation by a novel fungal oxalate oxidase from Abortiporus biennis

Marcin Grąz; Kamila Rachwał; Radosław Zan; Anna Jarosz-Wilkołazka

doi:10.18388/abp.2016_1282

Oxalic acid degradation by a novel fungal oxalate oxidase from Abortiporus biennis

Marcin Grąz
Kamila Rachwał
Radosław Zan
Anna Jarosz-Wilkołazka

DOI: https://doi.org/10.18388/abp.2016_1282

Abstract

Oxalate oxidase was identified in mycelial extracts of a basidiomycete Abortiporus biennis strain. Intracellular enzyme activity was detected only after prior lowering of the pH value of the fungal cultures by using oxalic or hydrochloric acids. This enzyme was purified using size exclusion chromatography (Sephadex G-25) and ion-exchange chromatography (DEAE-Sepharose). This enzyme exhibited optimum activity at pH 2 when incubated at 40°C, and the optimum temperature was established at 60°C. Among the tested organic acids, this enzyme exhibited specificity only towards oxalic acid. Molecular mass was calculated as 58 kDa. The values of K_m for oxalate and V_max for the enzyme reaction were 0.015 M and 30 mmol min^-1, respectively.

Published

2016-06-23

Issue

Vol. 63 No. 3 (2016)

Section

Articles

Acta Biochimica Polonica is an OpenAccess quarterly and publishes four issues a year. All contents are distributed under the Creative Commons Attribution-ShareAlike 4.0 International (CC BY 4.0) license. Everybody may use the content following terms: Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.