Invasive Cx43high sub-line of human prostate DU145 cells displays increased nanomechanical deformability

Katarzyna Piwowarczyk; Michal Sarna; Damian Ryszawy; Jarosław Czyż

doi:10.18388/abp.2017_1593

Katarzyna Piwowarczyk Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University
Michal Sarna Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Cracow, Poland; Department of Medical Physics and Biophysics, Faculty of Physics and Applied Computer Science, AGH University of Science and Technology, Cracow, Poland
Damian Ryszawy Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University
Jarosław Czyż Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University

DOI: https://doi.org/10.18388/abp.2017_1593

Keywords: prostate cancer invasion, Cx43, cell elasticity, motility, AFM,

Abstract

Connexin(Cx)43^high cells are preferentially recruited to the invasive front of prostate cancer in vitro and in vivo. To address the involvement of Cx43 in the regulation of human prostate cancer DU145 cell invasiveness, we analysed the nanoelasticity of invasive Cx43^high sub-sets of DU145 cells by atomic force microscopy (AFM). Cx43^highDU145 cells displayed considerably higher susceptibility to mechanical distortions than the wild type DU145 cells. Transient Cx43 silencing had no effect on their elastic properties. Our data confirm the relationship between invasive potential, Cx43 expression and nanomechanical elasticity of DU145 cells. However, they also show that Cx43 is not directly involved in the maintenance of DU145 invasive phenotype.

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