The phenomenon of fluorescence in immunosensors.

Aleksandra Kłos-Witkowska

doi:10.18388/abp.2015_1231

The phenomenon of fluorescence in immunosensors.

Aleksandra Kłos-Witkowska Faculty of Mechanical Engineering and Computer Science, Department of Computer Science and Automatics, University of Bielsko-Biala, Bielsko-Biala, Poland.;

DOI: https://doi.org/10.18388/abp.2015_1231

Abstract

The phenomenon of fluorescence in immunosensors is described in this paper. Both structure and characteristics of biosensors and immunosensors are presented. Types of immunosensors and the response of bioreceptor layers to the reaction with analytes as well as measurements of electrochemical, piezoelectric and optical parameters in immunosensors are also presented. In addition, detection techniques used in studies of optical immunosensors based on light-matter interactions (absorbance, reflectance, dispersion, emission) such as: UV/VIS spectroscopy, reflectometric interference spectroscopy (RIfs), surface plasmon resonance (SPR), optical waveguide light-mode spectroscopy (OWLS), fluorescence spectroscopy. The phenomenon of fluorescence in immunosensors and standard configurations of immunoreactions between an antigen and an antibody (direct, competitive, sandwich, displacement) is described. Fluorescence parameters taken into account in analyses and fluorescence detection techniques used in research of immunosensors are presented. Examples of immunosensor applications are given.

Published

2016-05-19

Issue

Vol. 63 No. 2 (2016)

Section

Articles

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