Dietary resistant dextrins positively modulate fecal and cecal microbiota composition in young rats.

Katarzyna Śliżewska; Zdzisława Libudzisz; Renata Barczyńska; Janusz Kapuśniak; Zenon Zduńczyk; Jerzy Juśkiewicz

Katarzyna Śliżewska Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Lodz, Poland.;
Zdzisława Libudzisz Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Lodz, Poland.;
Renata Barczyńska Institute of Chemistry, Environmental Protection and Biotechnology, Jan Dlugosz University in Czestochowa, Czestochowa, Poland.;
Janusz Kapuśniak Institute of Chemistry, Environmental Protection and Biotechnology, Jan Dlugosz University in Czestochowa, Czestochowa, Poland.;
Zenon Zduńczyk Insitute of Animal Reproduction and Food Research, Polish Academy of Science in Olsztyn, Olsztyn, Poland.;
Jerzy Juśkiewicz Insitute of Animal Reproduction and Food Research, Polish Academy of Science in Olsztyn, Olsztyn, Poland.;

Abstract

The objective of the present study was to demonstrate the effect of dietary resistant dextrins, as potential prebiotics, on the intestinal microflora of young rats. Enzyme-resistant dextrin, prepared by heating of potato starch in the presence of hydrochloric (0.1% dsb) and tartaric (40% dsb) acid at 130ºC for 2 h (CA-dextrin). The experiment was performed on 24 Wistar male rats at 3-wk of age, divided by analogues in three experimental groups (control, starch and dextrin). Analyses determined the overall bacterial counts and the counts of Lactobacillus, Bifidobacterium, Bacteroides and Clostridium strains within the feces and cecal contents of rats using fluorescence in situ hybridization method. CA-dextrin had no effect on primary growth indicators (body weight, body weight gain, dietary consumption) or the mass of the small intestine and the cecum, but dextrins caused a reduction in pH and the concentration of ammonia within the cecal contents. That supplementation of diet with resistant dextrins had a positive effect on composition of intestinal microflora in rats. It increased the counts of Bifidobacterium and Lactobacillus strains both in the feces and in the cecum. Moreover, it reduced the counts of Clostridium and Bacteroides strains. These results may suggest that resistant dextrins exerted a prebiotic-like effect in the large intestine.