Inhibition of cell proliferation and induction of apoptosis in K562 human leukemia cells by the derivative (3-NpC) from dihydro-pyranochromenes family.

Roghayeh Rahimi; Majid Mahdavi; Sina Pejman; Payman Zare; Saeed Balalaei

doi:10.18388/abp.2014_825

Roghayeh Rahimi Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran.;
Majid Mahdavi Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran.;
Sina Pejman Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran.;
Payman Zare Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz. Iran.;
Saeed Balalaei Department of Chemistry, Faculty of Science, K. N. Toosi University of Tech, Tehran, Iran.;

DOI: https://doi.org/10.18388/abp.2014_825

Abstract

Leukemia is a particular type of cancer characterized by the failure of cell death or disability in differentiation of hematopoietic cells. Chronic myelogenus leukemia (CML) is the most studied kind of this cancer. In this study, anti-cancer effect of dihydro-pyranochromenes derivatives were investigated in the human leukemia K562 cells. These compounds were found to be active cell proliferation inhibitors using MTT assay. Among these compounds, 3-NpC was determined as stronger compound with IC50 value of 100 ± 3.1 µM and was chosen for further studies. Induction of apoptosis was analyzed by AO/EtBr staining, DNA fragmentation assay, Annexin V/PI double staining and cell cycle analysis. Furthermore, Western Blot analysis showed that treatment of the cells with 3-NpC led to up-regulation and activation of caspase-3. The results of this investigation clearly indicated that dihydro-pyranochromenes derivatives induce apoptosis in the K562 cell line. This information signalizes also that these compounds may prepare a new therapeutic approach for the treatment of leukemia.