Differentiation by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) of Candida albicans isolated from upper respiratory tract in patients with non-small cell lung cancer.

Anna Biernasiuk; Izabela Korona-Głowniak; Agnieszka Grzegorczyk; Anna Malm

doi:10.18388/abp.2014_1837

Differentiation by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) of Candida albicans isolated from upper respiratory tract in patients with non-small cell lung cancer.

Anna Biernasiuk Department of Pharmaceutical Microbiology, Medical University of Lublin, Lublin, Poland.;
Izabela Korona-Głowniak Department of Pharmaceutical Microbiology, Medical University of Lublin, Lublin, Poland.;
Agnieszka Grzegorczyk Department of Pharmaceutical Microbiology, Medical University of Lublin, Lublin, Poland.;
Anna Malm Department of Pharmaceutical Microbiology, Medical University of Lublin, Lublin, Poland.;

DOI: https://doi.org/10.18388/abp.2014_1837

Abstract

Cancer patients are predisposed to fungal infections caused by Candida albicans, especially to oral or respiratory tract candidiasis. The aim of this study was to estimate genetic diversity by RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) of C. albicans isolated from upper respiratory tract of 100 patients with non-small cell lung cancer. Among 52 strains, 34 genotypes were defined. 10 clusters comprising 28 (53.85%) isolates with similarity coefficient ≥ 80% were formed. The remaining 24 (46.15%) isolates represented individual genotypes. The RAPD-PCR technique revealed genomic variability within C. albicans isolated from upper respiratory tract of the cancer patients.

Published

2014-11-06

Issue

Vol. 61 No. 4 (2014)

Section

Articles

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