The antioxidant quercetin protects HL-60 cells with high myeloperoxidase activity against pro-oxidative and apoptotic effects of etoposide.

Monika A Papież; Wirginia Krzyściak

doi:10.18388/abp.2014_1848

The antioxidant quercetin protects HL-60 cells with high myeloperoxidase activity against pro-oxidative and apoptotic effects of etoposide.

Monika A Papież Department of Cytobiology, Faculty of Pharmacy, Jagiellonian University, Medical College, Kraków, Poland.;
Wirginia Krzyściak Department of Medical Diagnostics, Jagiellonian University, Medical College, Faculty of Pharmacy, Kraków, Poland.;

DOI: https://doi.org/10.18388/abp.2014_1848

Abstract

The protective action of quercetin against the pro-oxidant and apoptotic effect of etoposide was investigated in HL-60 cells with a high level of myeloperoxidase (MPO) activity and in cells treated with MPO inhibitor, 4-aminobenzoic acid hydrazide (ABAH). Quercetin significantly protected MPO-rich cells against the pro-oxidative (p<0.05) and apoptotic (p<0.05) effects of etoposide. Pre-treatment with ABAH abolished this protective influence of quercetin on apoptosis induced by etoposide but actually enhanced the action effect of quercetin against etoposide-generated reactive oxygen species (ROS) level by this cytostatic drug. Thus quercetin can protect HL-60 cells against the pro-oxidative activity of etoposide regardless of MPO activity.

Published

2014-12-11

Issue

Vol. 61 No. 4 (2014)

Section

Articles

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