High-grade mutant OmpF induces decreased bacterial survival rate.

Zhi-ping Zhao; Ting-ting Liu; Li Zhang; Min Luo; Xin Nie; Zai-xin Li; Yu Pan

doi:10.18388/abp.2014_1908

Zhi-ping Zhao School of Chemistry and Pharmaceutical Engineering, Sichuan University of Science & Engineering, Zigong, China.;
Ting-ting Liu School of Chemistry and Pharmaceutical Engineering, Sichuan University of Science & Engineering, Zigong, China.;
Li Zhang School of Chemistry and Pharmaceutical Engineering, Sichuan University of Science & Engineering, Zigong, China.;
Min Luo Journals Department, Chongqing University, Chongqing, China.;
Xin Nie School of Chemistry and Pharmaceutical Engineering, Sichuan University of Science & Engineering, Zigong, China.;
Zai-xin Li School of Chemistry and Pharmaceutical Engineering, Sichuan University of Science & Engineering, Zigong, China.;
Yu Pan College of Horticulture and Landscape Agriculture, Southwest University, Beibei, China.;

DOI: https://doi.org/10.18388/abp.2014_1908

Abstract

OmpF plays very important roles in the influx of antibiotics and bacterial survival in the presence of antibiotics. However, high-grade mutant OmpF and its function in decreasing bacterial survival rate have not been reported to date. In the present study, we cloned a high-grade mutant OmpF (mOmpF) and sequence analysis suggested that over 45 percent of the DNA sequence was significantly mutated, leading to dramatic changes in over 55 percent of the amino acid sequence. mOmpF protein was successfully expressed. When grown in the presence of antibiotic, the bacterial survival rate decreased and the antibiotic inhibition zone became larger with the increase of the mOmpF. It was concluded that concentration of high-grade mutant mOmpF dramatically influenced the bacterial survival rate. The study presented here may provide insights into better understanding of the relationships between structure and function of OmpF.