Determination of lysosomal exoglycosidases in human saliva.

Sylwia Chojnowska; Anna Zalewska; Małgorzata Knaś; Napoleon Waszkiewicz; Danuta Waszkiel; Agnieszka Kossakowska; Krzysztof Zwierz

doi:10.18388/abp.2014_1927

Sylwia Chojnowska Medical Institute, College of Computer Science and Business Administration, Lomza, Poland.;
Anna Zalewska Department of Conservative Dentistry, Medical University in Bialystok, Poland.;
Małgorzata Knaś The Institute of Health Care, The Higher Vocational School, Suwalki, Poland.;
Napoleon Waszkiewicz Department of Psychiatry, Medical University in Bialystok, Poland.;
Danuta Waszkiel Department of Conservative Dentistry, Medical University in Bialystok, Poland.;
Agnieszka Kossakowska Medical College of the Universal Education Society, Lomza, Poland.;
Krzysztof Zwierz Medical College of the Universal Education Society, Lomza, Poland.;

DOI: https://doi.org/10.18388/abp.2014_1927

Abstract

Currently we observe a growing interest in human saliva as a non-invasive material for diagnosis and monitoring of general and oral diseases. The aim of our study was adaptation of the Marciniak et al. (Marciniak J, Zalewska A, Popko J, Zwierz K, 2006, Clin Chem Lab Med 44: 933-937) method for determination of HEX and GLU activity in synovial fluid, and for determination of: HEX and GLU, as well as MAN, GAL, and FUC activity in human saliva. Under optimal conditions, 10 μl of saliva for HEX, and 30 μl for GLU, MAN, GAL and FUC, were sufficient for determination of human salivary exoglycosidases activity with variation coefficient ranging from 0.89 for GLU to 0.99 for GAL. The adapted method for exoglycosidases activity determination in human saliva is sufficiently sensitive and precise to use in clinical diagnosis.