Changes of DNA methylation and hydroxymethylation in plant protoplast cultures.

Pavla Moricová; Vladan Ondřej; Božena Navrátilová; Lenka Luhová

doi:10.18388/abp.2013_1947

Changes of DNA methylation and hydroxymethylation in plant protoplast cultures.

Pavla Moricová Department of Biochemistry, Faculty of Science, Palacýi University, Olomouc, Czech Republic.;
Vladan Ondřej
Božena Navrátilová
Lenka Luhová

DOI: https://doi.org/10.18388/abp.2013_1947

Abstract

Cytosine methylation patterns in higher eukaryotes are important in gene regulation. Along with 5-methylcytosine (5-mC), a newly discovered constituent of mammalian DNA, 5-hydroxymethylcytosine (5-hmC), is the other modified base in higher organisms. In this study we detected 5-hmC in plant protoplast DNA and demonstrated its increasing content during the first 72 hrs. of protoplast cultivation. In contrast to 5-hmC, the amount of 5-mC decreased during protoplast cultivation. It was also found that 5-hmC did not primarily arise as a product of oxidative DNA damage following protoplast culture.

Published

2013-03-15

Issue

Vol. 60 No. 1 (2013)

Section

Articles

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