Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis.

Jindřich Bříza; Daniela Pavingerová; Josef Vlasák; Hana Niedermeierová

doi:10.18388/abp.2013_1998

Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis.

Jindřich Bříza Institute of Plant Molecular Biology, Biology Centre AS CR, and Faculty of Science, South Bohemia University, České Budějovice, Czech Republic.;
Daniela Pavingerová
Josef Vlasák
Hana Niedermeierová

DOI: https://doi.org/10.18388/abp.2013_1998

Abstract

Modified versions of the Cry3A gene of Bacillus thuringiensis (Bt) were transferred into Norway spruce (Picea abies). Both the biolistic approach and Agrobacterium tumefaciens mediated procedure were employed for transformation of embryogenic tissue (ET) cultures. The latter method proved to be more efficient yielding 70 transgenic embryogenic tissue lines compared with 18 lines obtained by biolistics. The modified Cry3A genes were driven by a 35S promoter and the nptII screenable selection marker gene was used in all vectors. The transgenic ETs were molecularly characterized and converted into mature somatic embryos. Germinating embryos formed plantlets which were finally planted into perlite and their Cry3A gene transcription activities were demonstrated by RT-PCR.

Published

2013-07-25

Issue

Vol. 60 No. 3 (2013)

Section

Articles

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