A simple method for the determination of the cholesterol esterase activity.

Agnieszka Ewa Stępień; Mykhailo Gonchar

doi:10.18388/abp.2013_1999

A simple method for the determination of the cholesterol esterase activity.

Agnieszka Ewa Stępień Department of Biotechnology, Institute of Applied Biotechnology and Basic Science; and Centre of Applied Biotechnology and Basic Sciences, University of Rzeszow, Kolbuszowa, Poland.;
Mykhailo Gonchar

DOI: https://doi.org/10.18388/abp.2013_1999

Abstract

The proposed method determines the activity of cholesterol esterase (CEH) and takes advantage of its ability to catalyze the hydrolysis of cholesterol esters naturally present in human serum. The assay is based on Allain's method of spectrophotometric determination of cholesterol by means of cholesterol oxidase, peroxidase, but using 3,5-dichloro-dihydroxybenzenesulfonic acid (DHBS) as phenolic chromogen and human serum as a source of substrate for the CEH as a novelty. Furthermore, it is characterized by low costs and high precision. It can be employed to control the activity of CE preparations used for the preparation of enzymatic kits for the determination of cholesterol or for screening of potential bacterial enzyme producers.

Published

2013-09-20

Issue

Vol. 60 No. 3 (2013)

Section

Articles

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