Generation of stable, non-aggregating Saccharomyces cerevisiae wild isolates.

Dominika M Wloch-Salamon; Marcin Plech; Jagoda Majewska

doi:10.18388/abp.2013_2036

Generation of stable, non-aggregating Saccharomyces cerevisiae wild isolates.

Dominika M Wloch-Salamon Institute of Environmental Sciences, Jagiellonian University, Kraków, Poland.;
Marcin Plech Institute of Environmental Sciences, Jagiellonian University, Kraków, Poland.;
Jagoda Majewska Institute of Environmental Sciences, Jagiellonian University, Kraków, Poland.;

DOI: https://doi.org/10.18388/abp.2013_2036

Abstract

Cellular aggregates observed during growth of Saccharomyces cerevisiae strains derived from various natural environments makes most laboratory techniques optimized for non-aggregating laboratory strains inappropriate. We describe a method to reduce the size and percentage of the aggregates. This is achieved by replacing the native allele of the AMN1 gene with an allele found in the W303 laboratory strain. The reduction in aggregates is consistent across various environments and generations, with no change in maximum population density or strain viability, and only minor changes in maximum growth rate and colony morphology.

Published

2013-12-20

Issue

Vol. 60 No. 4 (2013)

Section

Articles

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