Differences in metabolic profiles of planktonic and biofilm cells in Staphylococcus aureus - (1)H Nuclear Magnetic Resonance search for candidate biomarkers.

Adam Feliks Junka; Stanisław Deja; Danuta Smutnicka; Patrycja Szymczyk; Grzegorz Ziółkowski; Marzenna Bartoszewicz; Piotr Młynarz

doi:10.18388/abp.2013_2044

Adam Feliks Junka Department of Microbiology, Medical University of Wroclaw, Wrocław, Poland.;
Stanisław Deja Faculty of Chemistry, Opole University, Opole, Poland.;
Danuta Smutnicka Department of Microbiology, Medical University of Wroclaw, Wrocław, Poland.;
Patrycja Szymczyk Wroclaw University of Technology, Centre for Advanced Manufacturing Technologies, Wrocław, Poland.;
Grzegorz Ziółkowski Wroclaw University of Technology, Centre for Advanced Manufacturing Technologies, Wrocław, Poland.;
Marzenna Bartoszewicz Department of Microbiology, Medical University of Wroclaw, Wrocław, Poland.;
Piotr Młynarz Faculty of Chemistry, Opole University, Opole, Poland.;

DOI: https://doi.org/10.18388/abp.2013_2044

Abstract

Staphylococcus aureus is responsible for many types of infections related to biofilm presence. As the early diagnostics remains the best option for prevention of biofilm infections, the aim of the work presented was to search for differences in metabolite patterns of S. aureus ATCC6538 biofilm vs. free-swimming S. aureus planktonic forms. For this purpose, Nuclear Magnetic Resonance (NMR) spectroscopy was applied. Data obtained were supported by means of Scanning Electron Microscopy, quantitative cultures and X-ray computed microtomography. Metabolic trends accompanying S. aureus biofilm formation were found using Principal Component Analysis (PCA). Levels of isoleucine, alanine and 2,3-butanediol were significantly higher in biofilm than in planktonic forms, whereas level of osmoprotectant glycine-betaine was significantly higher in planktonic forms of S. aureus. Results obtained may find future application in clinical diagnostics of S. aureus biofilm-related infections.