Genetic polymorphism of Hucul horse population based on 17 microsatellite loci.

  • Agnieszka Fornal National Research Institute of Animal Production, Department of Cytogenetics and Molecular Genetics of Animals, Balice n. Kraków, Poland.;
  • Anna Radko National Research Institute of Animal Production, Department of Cytogenetics and Molecular Genetics of Animals, Balice n. Kraków, Poland.;
  • Agata Piestrzyńska-Kajtoch National Research Institute of Animal Production, Department of Cytogenetics and Molecular Genetics of Animals, Balice n. Kraków, Poland.;
DOI: https://doi.org/10.18388/abp.2013_2054

Abstract

Short tandem repeat (STR) loci, i.e. microsatellites are a class of genetic markers commonly used for population studies and parentage control. This study determined the usefulness of microsatellite markers recommended by International Society for Animal Genetics (ISAG) for identification and pedigree analysis in horses based on the example of Polish Hucul horse population (Equus caballus). The set of seventeen microsatellites loci was tested (AHT4, AHT5, ASB2, HMS2, HMS3, HMS6, HMS7, HTG10, HTG4, HTG6, HTG7, VHL20, ASB17, ASB23, CA425, HMS1, LEX3) for 216 individuals. All samples were genotyped and mean number of alleles per locus was estimated (7.00). Means of observed (Ho) and expected (He) heterozygosity were calculated 0.7288 and 0.7027, respectively. The observed heterozygosity was similar to the results of research on Hucul horse population in another area of Carpathians Mountains. The average polymorphism information content (PIC) for analyses of seventeen microsatellite markers indicates the usefulness of this set of markers for Hucul horse parentage testing.
Issue
Vol. 60 No. 4 (2013)
Section
Articles

Acta Biochimica Polonica is an OpenAccess quarterly and publishes four issues a year. All contents are distributed under the Creative Commons Attribution-ShareAlike 4.0 International (CC BY 4.0) license. Everybody may use the content following terms: Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

Copyright for all published papers © stays with the authors.

Copyright for the journal: © Polish Biochemical Society.