Micelle-mediated extraction of elderberry blossom by whey protein and naturally derived surfactants.

  • Karolina Śliwa Faculty of Chemical Engineering and Technology, Cracow University of Technology, Kraków, Poland.;
  • Anna Tomaszkiewicz-Potępa Faculty of Chemical Engineering and Technology, Cracow University of Technology, Kraków, Poland.;
  • Elżbieta Sikora Faculty of Chemical Engineering and Technology, Cracow University of Technology, Kraków, Poland.;
  • Jan Ogonowski Faculty of Chemical Engineering and Technology, Cracow University of Technology, Kraków, Poland.;

Abstract

Classical methods of the extraction of active ingredients from the plant material are expensive, complicated and often environmentally unfriendly. The micelle-mediated extraction method (MME) seems to be a good alternative. In this work, extractions of elderberry blossoms (Flos Sambuci) were performed using MME methods. Several popular surfactants and whey protein concentrate (WPC) was applied in the process. The obtained results were compared with those obtained in extraction by means of water. Antioxidant properties of the extracts were analyzed by using two different methods: reaction with di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) reagent and Follin's method. Furthermore, the flavonoid content in the extracts was determined. The results confirmed that the MME method with using whey protein might be an alternative method for obtaining, rich in natural antioxidants, plant extracts.
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