Dexamethasone inhibits U937 cell adhesion via the down-regulation of ROCK1 activity.

Dong Liu; Xing-Yun Chen; Ren-Ping Xiong; Ya-Lei Ning; Ping Li; Yan Peng; Ping Liu; Yan Zhao; Nan Yang; Yuan-Guo Zhou

doi:10.18388/abp.2012_2091

Dexamethasone inhibits U937 cell adhesion via the down-regulation of ROCK1 activity.

Dong Liu Molecular Biology Center, State Key Laboratory of Trauma, Burn, and Combined Injury, Research Institute of Surgery and Daping Hospital, Third Military Medical University, Chongqing 400042, China.;
Xing-Yun Chen
Ren-Ping Xiong
Ya-Lei Ning
Ping Li
Yan Peng
Ping Liu
Yan Zhao
Nan Yang
Yuan-Guo Zhou

DOI: https://doi.org/10.18388/abp.2012_2091

Abstract

To explore the effect of dexamethasone (DEX) on monocyte adhesion function and its underlying mechanism. The effects of DEX and fasudil on adhesion of cultured U937 monocytes to human umbilical vein endothelial cells (HUVEC) following stimulation with phorbol myristate acetate (PMA) were studied; Changes in the Rho-associated coiled-coil protein kinase 1 (ROCK1) protein content and activity were evaluated. DEX and fasudil significantly inhibited U937 cell adhesion rates under PMA stimulation and inhibited ROCK1 activity. Mifepristone (RU-486) and cycloheximide (CHX) did not alter these effects of DEX. DEX interferes with the adhesion function of U937 cells through the inhibition of ROCK1 activity.

Published

2012-10-16

Issue

Vol. 59 No. 4 (2012)

Section

Articles

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