A rapid and simple method for detection of type II restriction endonucleases in cells of bacteria with high activity of nonspecific nucleases.

Beata Podgórska; Grażyna Kujawska; Michał Skurzewski; Olesya Batsko; Tadeusz Kaczorowski

doi:10.18388/abp.2012_2108

A rapid and simple method for detection of type II restriction endonucleases in cells of bacteria with high activity of nonspecific nucleases.

Beata Podgórska Department of Molecular Evolution, University of Gdańsk, Gdańsk, Poland.;
Grażyna Kujawska
Michał Skurzewski
Olesya Batsko
Tadeusz Kaczorowski

DOI: https://doi.org/10.18388/abp.2012_2108

Abstract

In this work we describe a novel, rapid and simple microscale procedure for identification of restriction endonuclease activity in bacteria lysates, which contain high levels of non-specific DNA nucleases.

Published

2012-11-06

Issue

Vol. 59 No. 4 (2012)

Section

Articles

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