Variations in xanthophyll composition in etiolated seedlings of Arabidopsis thaliana correlate with protochlorophyllide accumulation.

Beata Myśliwa-Kurdziel; Małgorzata Jemioła-Rzemińska; Elżbieta Turek; Kazimierz Strzałka; Przemysław Malec

doi:10.18388/abp.2012_2171

Variations in xanthophyll composition in etiolated seedlings of Arabidopsis thaliana correlate with protochlorophyllide accumulation.

Beata Myśliwa-Kurdziel Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.;
Małgorzata Jemioła-Rzemińska
Elżbieta Turek
Kazimierz Strzałka
Przemysław Malec

DOI: https://doi.org/10.18388/abp.2012_2171

Abstract

Protochlorophyllide (Pchlide) accumulation and xantophyll composition were studied in 5-day old etiolated seedlings of three ecotypes of Arabidopsis thaliana: Columbia (Col-0), Landsberg erecta (Ler) and Wassiliewska (Ws). The total Pchlide level as measured by fluorescence spectroscopy varied significantly between ecotypes. A rapid HPLC method revealed quantitative differences in carotenoid composition. It was found that in the Ler ecotype any enhanced accumulation of Pchlide correlates with an increased level of lutein, suggesting the role of enzymes involved in lutein synthesis in cross-regulation between chlorophyll and carotenoid biosynthetic pathways. The function of the dark-accumulated carotenoid pool in seedling de-etiolation is discussed.

Published

2012-03-17

Issue

Vol. 59 No. 1 (2012)

Section

Articles

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