A novel alkaline protease with antiproliferative activity from fresh fruiting bodies of the toxic wild mushroom Amanita farinosa.

Jian Sun; Yongchang Zhao; Hongmei Chai; Hexiang Wang; Tzi Bun Ng

doi:10.18388/abp.2011_2226

A novel alkaline protease with antiproliferative activity from fresh fruiting bodies of the toxic wild mushroom Amanita farinosa.

Jian Sun State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing, China.;
Yongchang Zhao
Hongmei Chai
Hexiang Wang
Tzi Bun Ng

DOI: https://doi.org/10.18388/abp.2011_2226

Abstract

A novel protease with a molecular mass of 15 kDa was purified from fresh fruiting bodies of the wild mushroom Amanita farinosa. The purification protocol entailed anion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, cation exchange chromatography on SP-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. The protease was unadsorbed on DEAE-cellulose but adsorbed on Affi-gel blue gel and SP-Sepharose. It demonstrated a single 15-kDa band in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS/PAGE) and a 15-kDa peak in gel filtration. The optimal pH and optimal temperature of the protease were pH 8.0 and 65 °C, respectively. Proliferation of human hepatoma HepG2 cells was inhibited by the protease with an IC(50) of 25 µM. The protease did not have antifungal or ribonuclease activity.

Published

2011-12-06

Issue

Vol. 58 No. 4 (2011)

Section

Articles

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