In vitro inhibition of topoisomerase IIα by reduced glutathione.

Zahid M Delwar; Marina Fernanda Vita; Åke Siden; Mabel Cruz; Juan Sebastian Yakisich

doi:10.18388/abp.2011_2276

In vitro inhibition of topoisomerase IIα by reduced glutathione.

Zahid M Delwar Department of Clinical Neuroscience, Karolinska Institute, Stockholm, Sweden.;
Marina Fernanda Vita
Åke Siden
Mabel Cruz
Juan Sebastian Yakisich

DOI: https://doi.org/10.18388/abp.2011_2276

Abstract

In most cells, the major intracellular redox buffer is glutathione (GSH) and its disulfide-oxidized (GSSG) form. The GSH/GSSG system maintains the intracellular redox balance and the essential thiol status of proteins by thiol disulfide exchange. Topoisomerases are thiol proteins and are a target of thiol-reactive substances. In this study, the inhibitory effect of physiological concentration of GSH and GSSG on topoisomerase IIα activity in vitro was investigated. GSH (0-10 mM) inhibited topoisomerase IIα in a concentration-dependent manner while GSSG (1-100 µM) had no significant effect. These findings suggest that the GSH/GSSG system could have a potential in vivo role in regulating topoisomerase IIα activity.

Published

2011-06-06

Issue

Vol. 58 No. 2 (2011)

Section

Articles

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