New method for the study of Amaryllidaceae alkaloid biosynthesis using biotransformation of deuterium-labeled precursor in tissue cultures.

Anna El Tahchy; Michel Boisbrun; Agata Ptak; François Dupire; Françoise Chrétien; Max Henry; Yves Chapleur; Dominique Laurain-Mattar

doi:10.18388/abp.2010_2375

New method for the study of Amaryllidaceae alkaloid biosynthesis using biotransformation of deuterium-labeled precursor in tissue cultures.

Anna El Tahchy Groupe S.U.C.R.E.S., UMR 7565 CNRS-Nancy-Université, Nancy-Vandoeuvre, France.;
Michel Boisbrun
Agata Ptak
François Dupire
Françoise Chrétien
Max Henry
Yves Chapleur
Dominique Laurain-Mattar

DOI: https://doi.org/10.18388/abp.2010_2375

Abstract

Biotransformation of deuterated-4'-O-methylnorbelladine into alkaloids galanthamine and lycorine in tissue cultures of Leucojum aestivum was demonstrated using HPLC coupled to mass spectrometry. GC-MS screening was also carried to investigate other native and deuterated alkaloids. A total of six labeled alkaloids were identified indicating that 4'-O-methyl-d(3)-norbelladine is incorporated into three different groups of Amaryllidaceae alkaloids that are biosynthesized by three modes of intramolecular oxidative phenol coupling.

Published

2010-01-11

Issue

Vol. 57 No. 1 (2010)

Section

Articles

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