One-step purification of vitronectin from human plasma by affinity chromatography on phage-displayed peptides.

Szymon Skurzynski

doi:10.18388/abp.2010_2377

One-step purification of vitronectin from human plasma by affinity chromatography on phage-displayed peptides.

Szymon Skurzynski Department of Molecular and Medical Biophysics, Medical University of Łódź, Łódź, Poland. szymon.skurzynski@umed.lodz.pl;

DOI: https://doi.org/10.18388/abp.2010_2377

Abstract

A novel affinity purification method for rapid isolation of vitronectin (VN) from human plasma is described. Recently we have used phage display technology to obtain clones expressing peptides with high binding activity for VN. The isolated "strong VN binders" were covalently coupled to CNBr-activated Sepharose. Human plasma was applied to the column and bound VN was eluted using 0.5 M acetic acid, giving purity exceeding 90%. The developed method is a convenient alternative to conventional antibody-antigen affinity chromatography techniques for purification of VN, as it offers low ligand cost, is rapid and ensures good protein recovery from human plasma.

Published

2010-03-16

Issue

Vol. 57 No. 1 (2010)

Section

Articles

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