Antitumoral effect of IL-12 gene transfected via liposomes into B16F0 cells.

Lucía Speroni; Julieta Gasparri; Victoria de los A Bustuoabad; Nadia S Chiaramoni; Andrzej Smagur; Stanisław Szala; María C Taira; Silvia del V Alonso

doi:10.18388/abp.2009_2456

Antitumoral effect of IL-12 gene transfected via liposomes into B16F0 cells.

Lucía Speroni Laboratorio de Biomembranas, Departamento de Ciencia y Tecnologia, Universidad Nacional de Quilmes, Buenos Aires, Argentina.;
Julieta Gasparri
Victoria de los A Bustuoabad
Nadia S Chiaramoni
Andrzej Smagur
Stanisław Szala
María C Taira
Silvia del V Alonso

DOI: https://doi.org/10.18388/abp.2009_2456

Abstract

Murine melanoma B16F0 cells were transfected with SA:DPPC:DOPE (2:1:1 molar ratio) liposomes associated with a plasmid encoding murine IL-12. Stearylamine, a cationic lipid, showed a greater transfection efficiency compared to DOTAP-containing liposomes. The lipid:DNA ratio was 2:1 (w/w). Control groups were mock transfected or transfected with an empty plasmid (pNeo). pNeo or IL-12 transfected cells and controls were inoculated intradermically into the dorsal region of the foot or the lateral flank of C57BL6 mice. Results showed that IL-12 expression had a marked effect on in vivo growth of B16 melanoma tumors developed in both anatomic sites, significantly retarding their growth and prolonging host survival.

Published

2009-05-07

Issue

Vol. 56 No. 2 (2009)

Section

Articles

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