Diphenyl diselenide and diphenyl ditelluride increase the latency for 4-aminopyridine-induced chemical seizure and prevent death in mice.

Abstract

In this work was investigated the effect of pre-treatment with (PhSe)(2) and (PhTe)(2) on chemical seizure and 4-aminopyridine-induced lethality in mice. Additionally, lipid peroxidation levels of whole brain after treatment with 4-aminopyridine and effect of pre-treatment with (PhSe)(2) and (PhTe)(2) on these levels were investigated. Mice were pre-treated with (PhSe)(2) or (PhTe)(2) (50, 100, or 150 micromol/kg) 30 min before 4-aminopyridine (12 mg/kg) administration. The treatment with 4-aminopyridine caused a significant incidence of seizures (clonic, tonic) and death. Pre-treatment with (PhSe)(2) and (PhTe)(2) significantly increased the latency for clonic and tonic seizures, and prevented 4-aminopyridine-induced death. Significantly, the pre-treatment with (PhSe)(2) or (PhTe)(2) increased the latency for clonic seizures in a dose-dependent manner. Additionally, a significant increase was observed in the brain lipid peroxidation level after treatment with 4-aminopyridine, which was significantly inhibited by pre-treatment with 150 micromol/kg (PhSe)(2) or (PhTe)(2). These results demonstrate that (PhSe)(2) and (PhTe)(2) counteract the harmful effects of 4-aminopyridine. It is possible that this effect results from modulation of the redox state of N-methyl-d-aspartate receptors and/or of Ca(2+) channel activity with subsequent alteration in neurotransmitter release. Importantly, this study provides evidence for anticonvulsant and antioxidant properties of (PhSe)(2) and (PhTe)(2), which indicates a neuroprotective activity of these compounds.