Novel laccase-like multicopper oxidases from the fungus Myrothecium roridum - production enhancement, identification and application in dyes removal
Abstract
The aim of the study was to overproduce, identify and apply in dyes removal of a novel laccase-like multicopper oxidases (LMCOs) from Myrothecium roridum. LMCOs production was enhanced by modifying the medium and adding copper ions. After purification two proteins, LMCO1 and LMCO2, with molecular masses 46,7 and 66,3 kDa were discovered. The peptides analysis by mass spectrometry revealed that they belonged to the cupredoxin superfamily. Characteristic peptide sequences were obtained for MCOs and bilirubin oxidases. The crude enzymes were applied for dyes decolorization. Supplementation with 1 mM of vanillin allowed almost complete elimination of Indigo carmine within 3 hours. The dye was removed from solution containing metals, surfactants and organic solvents. The in-gel assessment of the activity and decolorization ability of MCOs followed by protein extraction and SDS-PAGE confirmed that only LMCO2 was responsible for the dye removal. MCOs produced by Myrothecium sp. have been poorly studied before. The obtained results increase the knowledge on this subject and may contribute to the development of an eco-friendly method of dyes elimination.
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