Cold-modulated small proteins abundance in winter triticale (x Triticosecale, Wittm.) seedlings tolerant to the pink snow mould (Microdochium nivale, Samuels and Hallett) infection

Gabriela Julia Golebiowska; Emilia Bonar; Kaveh Emami; Maria Wędzony

doi:10.18388/abp.2019_2804

Gabriela Julia Golebiowska Pedagogical University of Cracow, Institute of Biology, Department of Cell Biology and Genetics, Kraków, Poland
Emilia Bonar Jagiellonian University, Faculty of Biochemistry, Biophysics and Biotechnology, Department of Analytical Biochemistry, Kraków, Poland https://orcid.org/0000-0001-5532-8992
Kaveh Emami University of Newcastle Upon Tyne, School of Biology, Institute for Research on Environment and Sustainability, NE1 7RU, UK https://orcid.org/0000-0003-0182-0070
Maria Wędzony Pedagogical University of Cracow, Institute of Biology, Department of Cell Biology and Genetics, Kraków, Poland https://orcid.org/0000-0001-5911-2208

DOI: https://doi.org/10.18388/abp.2019_2804

Abstract

Two winter triticale (x Triticosecale Wittmack) model cultivars: Hewo (tolerant to pink snow mould) and Magnat (sensitive) were used to test the effect of cold-hardening (4 weeks at 4°C) on soluble ≤50 kDa protein profiles of the seedling leaves. The presence and abundance of individual proteins were analysed via two-dimensional gel electrophoresis (2-DE) and Surface-Enhanced Laser Desorption/Ionization Time-of-Flight (SELDI-TOF). Up to now, no proteomics analysis of triticale response to hardening has been performed. Thus, the present paper is the first in the series describing the obtained results. In our experiments, the exposure to the low temperature-induced only quantitative changes in the leaves of both cultivars, causing either an increase or decrease of 4–50 kDa protein abundance. Among proteins which were cold-accumulated in cv. Hewo’s leaves, we identified two thioredoxin peroxidases (chloroplastic thiol-specific antioxidant proteins) as well as mitochondrial- β-ATP synthase subunit and ADP-binding resistance protein. On the contrary, in hardened seedlings of this genotype, we observed the decreased level of chloroplastic RuBisCO small subunit PW9 and epidermal peroxidase 10. Simultaneous SELDI-TOF analysis revealed several low mass proteins better represented in cold-hardened plants of tolerant genotype in comparison to the sensitive one and the impact of both genotype and temperature on their level. Based on those results, we suggest that indicated proteins might be potential candidates for molecular markers of cold-induced snow mould resistance of winter triticale and their role is worth to be investigated in the further inoculation experiments.