Effect of ZnO, TiO2, Al2O3 and ZrO2 nanoparticles on wheat callus cells

Agnieszka Czyżowska; Anna Barbasz

doi:10.18388/abp.2019_2836

Effect of ZnO, TiO2, Al2O3 and ZrO2 nanoparticles on wheat callus cells

Agnieszka Czyżowska Department of Biochemistry, Biophysics and Biotechnology, Institute of Biology, Pedagogical University of Cracow, Kraków, Poland https://orcid.org/0000-0003-3398-4475
Anna Barbasz Department of Biochemistry, Biophysics and Biotechnology, Institute of Biology, Pedagogical University of Cracow, Kraków, Poland https://orcid.org/0000-0001-5210-2349

DOI: https://doi.org/10.18388/abp.2019_2836

Abstract

Effect of metal oxide nanoparticles on calli of two wheat varieties: Parabola (stress tolerant) and Raweta (sensitive) was studied. ZnO induced 10% larger membrane damage in Raweta calli. TiO2, Al2O3, and ZrO2 caused nearly 30% greater lactate dehydrogenase leakage for Raweta compared to Parabola. UV-irradiation of samples containing ZnO particles intensified this effect. Membrane lipid peroxidation in ZnO treated Raweta calli was twice as high as in Parabola and further increased after UV-irradiation. TiO2, Al2O3, and ZrO2 nanoparticles caused a 4-fold increase in malondialdehyde concentration in Raweta calli in comparison to Parabola calli. The nanoparticles studied damaged the cellular defense system by inactivating the antioxidative enzymes.

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Published

2019-09-17

Issue

Vol. 66 No. 3 (2019)

Section

Articles

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