Roundup-induced biochemical and histopathological changes in the liver and kidney of rats: the ameliorative effects of Linum usitatissimum oil

Nesrine  Djaber; Lynda Sabrina  Ounaceur; Baya Nouha  Moubine; Taha  Khaldi; Mereim  Rouag; Salma  Berrouague; Hichem  Amara; Faiza  Taibi; Mahieddine  Boumendjel; Amel Boumendjel; Mahfoud Messarah

doi:10.18388/abp.2020_2898

Nesrine Djaber Laboratory of Biochemistry and Environmental Toxicology, Faculty of Sciences, University of Badji Mokhtar, Annaba, Algeria https://orcid.org/0000-0002-3163-6058
Lynda Sabrina Ounaceur Laboratory of Biochemistry and Environmental Toxicology, Faculty of Sciences, University of Badji Mokhtar, Annaba, Algeria https://orcid.org/0000-0002-1455-566X
Baya Nouha Moubine Laboratory of Biochemistry and Environmental Toxicology, Faculty of Sciences, University of Badji Mokhtar, Annaba, Algeria
Taha Khaldi Laboratory of Biochemistry and Environmental Toxicology, Faculty of Sciences, University of Badji Mokhtar, Annaba, Algeria
Mereim Rouag Laboratory of Biochemistry and Environmental Toxicology, Faculty of Sciences, University of Badji Mokhtar, Annaba, Algeria
Salma Berrouague Laboratory of Biochemistry and Environmental Toxicology, Faculty of Sciences, University of Badji Mokhtar, Annaba, Algeria
Hichem Amara Laboratory of Biochemistry and Environmental Toxicology, Faculty of Sciences, University of Badji Mokhtar, Annaba, Algeria
Faiza Taibi
Mahieddine Boumendjel Laboratory of Biochemistry and Environmental Toxicology, Faculty of Sciences, University of Badji Mokhtar, Annaba, Algeria
Amel Boumendjel Laboratory of Biochemistry and Environmental Toxicology, Faculty of Sciences, University of Badji Mokhtar, Annaba, Algeria https://orcid.org/0000-0002-4278-2317
Mahfoud Messarah Laboratory of Biochemistry and Environmental Toxicology, Faculty of Sciences, University of Badji Mokhtar, Annaba, Algeria https://orcid.org/0000-0002-0928-0542

DOI: https://doi.org/10.18388/abp.2020_2898

Abstract

The present study was undertaken to evaluate the protective effects of Linum usitatissimum oil (LuO) against sub-chronic Roundup (RDP)-induced toxicity and oxidative stress in rats. Rats were divided into four groups: control group (no treatment), RDP group (Roundup at 269.9 mg/kg b.w.), LuO group (0.5 g/kg b.w. of LuO) and RDP+LuO group (RDP and LuO simultaneously). LuO decreased the ferric reducing antioxidant power (FRAP) (IC50=10.36 μg/ml) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50=22.85 mg/ml) in the tested tissues. The 30-day exposure of rats to RDP caused an increase in serum hepatic and renal markers: AST, ALT, ALP, LDH, γGT, bilirubin, urea, and creatinine. In addition, SOD, CAT and GST activities decreased by 43%, 61%, and 61%, respectively, while GPx activity, MDA and PCOs levels increased by 80%, 46%, 25%, respectively. LuO treatment alleviated hepatotoxicity in RDP-treated rats, showing improved levels of oxidative stress biomarkers and plasma biochemical parameters. The histological examination of the liver and kidney confirmed the changes in Roundup-treated rats and demonstrated the protective role of LuO.