Expression of soluble recombinant TGF-beta type II receptor fused with the Fc portion of human IgG1 (sTbetaRII-Fc) in NS0 cells.

Eliza P Kwiatkowska; Urszula Kazimierczak; Andrzej Mackiewicz; Dariusz W Kowalczyk

doi:10.18388/abp.2006_3350

Expression of soluble recombinant TGF-beta type II receptor fused with the Fc portion of human IgG1 (sTbetaRII-Fc) in NS0 cells.

Eliza P Kwiatkowska Department of Cancer Diagnostics and Immunology, GreatPoland Cancer Center, Poznań, Poland.;
Urszula Kazimierczak
Andrzej Mackiewicz
Dariusz W Kowalczyk

DOI: https://doi.org/10.18388/abp.2006_3350

Abstract

We have constructed and expressed recombinant chimeric soluble TGF-beta type II receptor fused with the Fc portion of human IgG1 (sTbetaRII-Fc) in NS0 mouse myeloma cells and isolated cell lines constitutively secreting very high levels of biologically active protein. The GS-NS0 expression system takes advantage of the strong human cytomegalovirus immediate early promoter expression vector and glutamine synthetase as a selectable marker. The recombinant chimeric receptor could be produced in high amounts and efficiently purified by one step chromatography on a protein A column. Biochemical studies revealed that recombinant sTbetaRII-Fc binds native TGF-beta1 and TGF-beta3 isoforms and neutralizes their activity in vitro.

Published

2006-05-30

Issue

Vol. 53 No. 2 (2006)

Section

Articles

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