Congo red interaction with alpha-proteins.

Jolanta Sereikaite; Vladas-Algirdas Bumelis

doi:10.18388/abp.2006_3366

Congo red interaction with alpha-proteins.

Jolanta Sereikaite Department of Chemistry and Bioengineering, Faculty of Fundamental Sciences, Vilnius Gediminas Technical University, Vilnius, Lithuania. sjolanta@fm.vtu.lt;
Vladas-Algirdas Bumelis

DOI: https://doi.org/10.18388/abp.2006_3366

Abstract

The ability of Congo red to form complexes with alpha-proteins, human growth hormone and human interferon-alpha2b, was found by absorption difference spectroscopy. A human growth hormone-Congo red complex was isolated by gel-permeation chromatography, and its visible absorption spectrum was registered in comparison to free dye. The ability of Congo red to induce dimerization of human growth hormone was demonstrated using chemical cross-linking agents 1,3,5-triacryloyl-hexahydro-s-triazine and ethylene glycol bis(succinimidylsuccinate).

Published

2006-03-30

Issue

Vol. 53 No. 1 (2006)

Section

Articles

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