Up-regulation of human PNPase mRNA by beta-interferon has no effect on protein level in melanoma cell lines.

Kamil Gewartowski; Rafal Tomecki; Lukasz Muchowski; Aleksandra Dmochow Ska; Artur Dzwonek; Michal Malecki; Henryk Skurzak; Jerzy Ostrowski; Piotr P Stepien

doi:10.18388/abp.2006_3376

Up-regulation of human PNPase mRNA by beta-interferon has no effect on protein level in melanoma cell lines.

Kamil Gewartowski Department of Genetics, Warsaw University, Warszawa, Poland.;
Rafal Tomecki
Lukasz Muchowski
Aleksandra Dmochow Ska
Artur Dzwonek
Michal Malecki
Henryk Skurzak
Jerzy Ostrowski
Piotr P Stepien

DOI: https://doi.org/10.18388/abp.2006_3376

Abstract

Human mitochondrial polynucleotide phosphorylase (hPNPase) is an exoribonuclease localized in mitochondria. The exact physiological function of this enzyme is unknown. Recent studies have revealed the existence of a relationship between induction of hPNPase mRNA and both cellular senescence and growth arrest of melanoma cells following beta-interferon treatment. The aim of this study was to verify whether the augmented hPNPase mRNA level results in increase of the protein level. In several cell lines established from five metastatic melanoma patients we did not find any such correlation. However, an elevated level of hPNPase protein was observed in interferon-induced HeLa and Jurkat cells. This increase was correlated with a slight shortening of poly(A) tails of mitochondrial ND3 transcript.

Published

2006-02-26

Issue

Vol. 53 No. 1 (2006)

Section

Articles

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