Isolation and expression pattern of RGS21 gene, a novel RGS member.

Xin Li; Lei Chen; Chaoneng Ji; Bing Liu; Jiefeng Gu; Jian Xu; Xianqiong Zou; Shaohua Gu; Yumin Mao

doi:10.18388/abp.2005_3412

Isolation and expression pattern of RGS21 gene, a novel RGS member.

Xin Li State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, People's Republic of China.;
Lei Chen
Chaoneng Ji
Bing Liu
Jiefeng Gu
Jian Xu
Xianqiong Zou
Shaohua Gu
Yumin Mao

DOI: https://doi.org/10.18388/abp.2005_3412

Abstract

Regulators of G-protein signaling (RGS) proteins are known for the RGS domain that is composed of a conserved stretch of 120 amino acids, which binds directly to activated G-protein alpha subunits and acts as a GTPase-activating protein (GAP), leading to their deactivation and termination of downstream signals. In this study, a novel human RGS cDNA (RGS21), 1795 bp long and encoding a 152-amino acid polypeptide, was isolated by large-scale sequencing analysis of a human fetal brain cDNA library. Unlike other RGS family members, RGS21 gene has no additional domain/motif and may represent the smallest known member of RGS family. It may belong to the B/R4 subfamily, which suggests that it may serve exclusively as a negative regulator of alphai/o family members and/or alphaq/11. PCR analysis showed that RGS21 mRNA was expressed ubiquitously in the 16 tissues examined, implying general physiological roles.

Published

2005-09-08

Issue

Vol. 52 No. 4 (2005)

Section

Articles

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