Characterization of Bacillus subtilis clones surviving overproduction of Zeta, a pSM19035 plasmid-encoded toxin.

Beata Nowakowska; Izabela Kern-Zdanowicz; Urszula Zielenkiewicz; Piotr Cegłowski

doi:10.18388/abp.2005_3492

Characterization of Bacillus subtilis clones surviving overproduction of Zeta, a pSM19035 plasmid-encoded toxin.

Beata Nowakowska Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland.;
Izabela Kern-Zdanowicz
Urszula Zielenkiewicz
Piotr Cegłowski

DOI: https://doi.org/10.18388/abp.2005_3492

Abstract

The postsegregational killing system of pSM19035 plasmid consists of the proteins Zeta and Epsilon, a toxin and an antidote, respectively. Zeta mutants were isolated with the use of Bacillus subtilis strain with the zeta gene under control of an inducible promoter integrated into the chromosome. Results of mutant analysis point to the amino terminal part of the Zeta protein as being responsible for the toxicity.

Published

2005-03-31

Issue

Vol. 52 No. 1 (2005)

Section

Articles

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