Restriction analysis of genetic variability of Polish isolates of Tomato black ring virus.

Magdalena Jończyk; Natasza Borodynko; Henryk Pospieszny

doi:10.18388/abp.2004_3552

Restriction analysis of genetic variability of Polish isolates of Tomato black ring virus.

Magdalena Jończyk Department of Virology and Bacteriology, Institute of Plant Protection, Poznań, Poland. m.palczewska@ior.poznan.pl;
Natasza Borodynko
Henryk Pospieszny

DOI: https://doi.org/10.18388/abp.2004_3552

Abstract

Several different isolates of Tomato black ring virus (TBRV) have been collected in Poland from cucumber, tomato, potato and black locust plants. Biological tests showed some differences in the range of infected plants and the type of symptoms, which was the basis for selection of seven the most biologically different TBRV isolates. According to the sequence of TBRV-MJ, several primer pairs were designed and almost the entire sequence of both genomic RNAs was amplified. The RT-PCR products derived from all tested TBRV isolates were digested by restriction enzymes. On the basis of the restriction patterns, the variable and the conserved regions of the TBRV genome were defined and the relationships between the Polish TBRV isolates established.

Published

2004-09-30

Issue

Vol. 51 No. 3 (2004)

Section

Articles

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