Overproduction and purification of the CcpA protein from Lactococcus lactis.

Magdalena Kowalczyk; Jacek Bardowski

doi:10.18388/abp.2003_3697

Overproduction and purification of the CcpA protein from Lactococcus lactis.

Magdalena Kowalczyk Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, A. Pawińskiego 5a, 02-106 Warsaw, Poland.;
Jacek Bardowski

DOI: https://doi.org/10.18388/abp.2003_3697

Abstract

In this work we present cloning and overexpression of lactococcal CcpA protein in Escherichia coli Xl1blue strain as a fusion with 6 x His tag. A high yield of the CcpA protein was obtained when the cells were cultured in liquid medium LB with 100 microg/ml ampicillin at 37 degrees C and subsequently for 4 h after induction by IPTG. The procedure let us obtain 5 mg of homogenous CcpA protein. Glutaraldehyde crosslinking analysis indicated the formation of dimer or tetramer forms of the CcpA protein.

Published

2003-06-30

Issue

Vol. 50 No. 2 (2003)

Section

Articles

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