Cellular level of 8-oxo-2'-deoxyguanosine in DNA does not correlate with urinary excretion of the modified base/nucleoside.

Marek Foksinski; Daniel Gackowski; Rafał Rozalski; Ryszard Olinski

doi:10.18388/abp.2003_3708

Cellular level of 8-oxo-2'-deoxyguanosine in DNA does not correlate with urinary excretion of the modified base/nucleoside.

Marek Foksinski Department of Clinical Biochemistry, The Ludwik Rydygier Medical University in Bydgoszcz, Karlowicza 24, 85-092 Bydgoszcz, Poland.;
Daniel Gackowski
Rafał Rozalski
Ryszard Olinski

DOI: https://doi.org/10.18388/abp.2003_3708

Abstract

We assessed a relationship between the level of 8-oxodG in leukocyte DNA measured with the high performance liquid chromatography with electrochemical detection (HPLC/EC) technique and urinary excretion of the modified nucleoside/base analysed with a recently developed methodology involving HPLC prepurification followed by gas chromatography with isotope dilution mass spectrometric detection. No correlation was found between these markers of oxidative DNA damage commonly used in epidemiological studies. Several possible explanations of this finding are discussed.

Published

2003-06-30

Issue

Vol. 50 No. 2 (2003)

Section

Articles

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