Interaction of maize (Zea mays) protein phosphatase 2A with tubulin.

Olubunmi S Awotunde; Katarzyna Lechward; Katarzyna Krajewska; Stanisław Zołnierowicz; Grazyna Muszyńska

doi:10.18388/abp.2003_3720

Interaction of maize (Zea mays) protein phosphatase 2A with tubulin.

Olubunmi S Awotunde Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland.;
Katarzyna Lechward
Katarzyna Krajewska
Stanisław Zołnierowicz
Grazyna Muszyńska

DOI: https://doi.org/10.18388/abp.2003_3720

Abstract

Immunological and biochemical evidence has been obtained for an interaction of maize protein phosphatase 2A (PP2A) holoenzyme with tubulin. Tubulin co-purifies with maize seedling PP2A. Affinity chromatography of the maize PP2A preparation on immobilized tubulin revealed two peaks of phosphorylase alpha phosphatase activity. In one of the peaks, the catalytic (C) and constant regulatory (A) subunits of PP2A were identified by Western blotting. The subunits (C and A) of PP2A were co-immunoprecipitated from maize seedlings homogenate by an anti-alpha-tubulin antibody. The interaction of plant PP2A with tubulin indicates a possible role of reversible protein phosphorylation in the dynamic structure of plant cytoskeleton.

Published

2003-03-31

Issue

Vol. 50 No. 1 (2003)

Section

Articles

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