High performance liquid chromatography and photodiode array detection of ferulic acid in Rubus protoplasts elicited by O-glycans from Fusarium sp. M7-1.

Mihai Nita-Lazar; Lionel Chevolot; Shojiro Iwahara; Kaoru Takegawa; Aleksandra Furmanek; Yvette Lienart

doi:10.18388/abp.2002_3762

High performance liquid chromatography and photodiode array detection of ferulic acid in Rubus protoplasts elicited by O-glycans from Fusarium sp. M7-1.

Mihai Nita-Lazar Centre de Recherches sur les Macromolécules Végétales-CNRS, associé à l'Université Joseph Fourier, Grenoble, France. mihain@micro.biol.ethz.ch;
Lionel Chevolot
Shojiro Iwahara
Kaoru Takegawa
Aleksandra Furmanek
Yvette Lienart

DOI: https://doi.org/10.18388/abp.2002_3762

Abstract

So far only little data have been available concerning the eliciting capacity of well defined glycan molecules isolated from plant pathogens. This study brings new information about changes in plant cells caused by fungal pathogens. Sugar fractions derived from glycoproteins isolated from the fungus Fusarium sp. M7-1 have been tested here as signaling molecules. The ability of three O-glycan fractions (named in this work inducer I, II, III) to trigger responses in Rubus protoplasts has been examined. It was found that inducer III was the most efficient as it elicited changes in the levels of phenylpropanoid pathway intermediates in relation to phenylalanine-ammonia lyase (PAL) activation.

Published

2002-12-31

Issue

Vol. 49 No. 4 (2002)

Section

Articles

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