Molecular cloning and sequencing of partial cDNA of tumor necrosis factor and p75 tumor necrosis factor receptor of Syrian golden hamster (Mesocricetus auratus) with the use of universal primers.

Zuzanna Dobrzańska; Joanna Wieckiewicz; Jacek Bigda

doi:10.18388/abp.2002_3801

Molecular cloning and sequencing of partial cDNA of tumor necrosis factor and p75 tumor necrosis factor receptor of Syrian golden hamster (Mesocricetus auratus) with the use of universal primers.

Zuzanna Dobrzańska Department of Histology and Immunology, Intercollegiate Faculty of Biotechnology UG-MUG, Medical University of Gdańsk, Poland.;
Joanna Wieckiewicz
Jacek Bigda

DOI: https://doi.org/10.18388/abp.2002_3801

Abstract

In this study we cloned and analysed partial cDNA of tumor necrosis factor (TNF) and p75 TNF-R receptor of Syrian golden hamster (Mesocricetus auratus). We obtained a 382-bp sequence of TNF and a 148-bp sequence coding for p75 TNF-R. The primers used for the cloning were designed on the basis of inter-species homology, thus presumably can be used for cloning and analysis of TNF and p75 TNF-R genes of other mammals.

Published

2002-06-30

Issue

Vol. 49 No. 2 (2002)

Section

Articles

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