Various cationic carriers for in vitro transfection of tumor and endothelial cell lines.

Barbara Zemlińska; Aleksander Sochanik; Ewa Missol-Kolka; Stanisław Szala

doi:10.18388/abp.2002_3847

Various cationic carriers for in vitro transfection of tumor and endothelial cell lines.

Barbara Zemlińska Department of Molecular Biology, Center of Oncology-Maria Skłodowska-Curie Memorial Institute, Gliwice, Poland.;
Aleksander Sochanik
Ewa Missol-Kolka
Stanisław Szala

DOI: https://doi.org/10.18388/abp.2002_3847

Abstract

We compared the efficiency of in vitro DNA transfer into selected tumor and endothelial cell lines using complexes of plasmid DNA and cationic carriers: DDAB/DOPE, DC-Chol/DOPE, Arg-Chol/DOPE, Gly-Chol/DOPE, Arg-Gly-Chol/DOPE, BGTC/DOPE, and PEI. The best carriers for transfecting the majority of tested cells lines at optimized carrier-to-DNA weight ratios were PEI and BGTC/DOPE.

Published

2002-03-31

Issue

Vol. 49 No. 1 (2002)

Section

Articles

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