Oligodeoxyadenylate stimulates the protein kinase activity of anti-DNA sIgA from human milk.

Yuri Kit; Elena Kuligina; Dimitry Semenov; Vladimir Richter

doi:10.18388/abp.2002_3848

Oligodeoxyadenylate stimulates the protein kinase activity of anti-DNA sIgA from human milk.

Yuri Kit Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv. kit@biochem.viv.ua;
Elena Kuligina
Dimitry Semenov
Vladimir Richter

DOI: https://doi.org/10.18388/abp.2002_3848

Abstract

Preparations of anti-DNA sIgA were obtained from human milk by sequential chromatography on protein A-sepharose, DEAE-fractogel and DNA-cellulose. The influence of oligonucleotides on protein kinase activity was investigated. It was discovered that incubation of anti-DNA sIgA with oligodeoxyriboadenylate d(A)12 stimulates the phosphorylation of polypeptides of sIgA in the presence of [gamma-32P]ATP. The greatest was the incorporation of P into the sIgA H-chains. We also demonstrated stimulation of the casein kinase activity of anti-DNA sIgA by d(A)12. The stimulation of the protein kinase activity of anti-DNA sIgA by oligoriboadenylate r(A)12 was not detected.

Published

2002-03-31

Issue

Vol. 49 No. 1 (2002)

Section

Articles

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