Micro-capillary liquid chromatography Fourier transform ion cyclotron resonance mass spectrometry--a powerful tool for peptide and protein identification.

  • M Ramström Department of Analytical Chemistry, Uppsala University, Sweden.;
  • M Palmblad
  • A Amirkhani
  • Y O Tsybin
  • K E Markides
  • P Håkansson
  • J Bergquist

Abstract

In order to be able to study complex biological samples, a micro-capillary liquid chromatography system was coupled to a 9.4 T Fourier transform ion cyclotron resonance mass spectrometer. The setup was tested on a tryptic digest of bovine serum albumin, which resulted in high sequence coverage (> 92%) of the protein.
Published
2001-12-31
Section
Articles