Detection and sequencing of new cyclic peptides from linseed by electrospray ionization mass spectrometry.

P Stefanowicz

doi:10.18388/abp.2001_3877

Detection and sequencing of new cyclic peptides from linseed by electrospray ionization mass spectrometry.

P Stefanowicz Faculty of Chemistry, University of Wrocław, Poland. stp@wchuwr.chem.uni.wroc.pl;

DOI: https://doi.org/10.18388/abp.2001_3877

Abstract

Extract of Linum usitatissimum seeds was analyzed by ESI-MS and ESI-MS/MS. The analysis confirms the presence of previously reported cyclolinopeptides: CLA (c(Pro-Pro-Phe-Phe-Leu-Ile-Ile-Leu-Val) and CLB (c(Pro-Pro-Phe-Phe-Val-Ile-Met-Ile-Leu)). Cyclolinopeptides CLD and CLE, which contain methionine oxide, were detected in small quantities only. These peptides likely result from the oxidation of their precursors, not reported previously: CLD' (c(Pro-Phe-Phe-Trp-Ile-Met-Leu-Leu)) and CLE'(c(Pro-Leu-Phe-Ile-Met-Leu-Val-Phe)), present at higher concentrations in unoxidized extract. Two new cyclic octapeptides: CLF (c(Pro-Phe-Phe-Trp-Val-Met-Leu-Met)) and CLG (c(Pro-Phe-Phe-Trp-Ile-Met-Leu-Met)) were detected and their sequences were proposed on the basis of CID experiments and similarity with those of CLD'.

Published

2001-12-31

Issue

Vol. 48 No. 4 (2001)

Section

Articles

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