Evaluation of high temperature glycation of proteins and peptides by electrospray ionization mass spectrometry.

P Stefanowicz; J Boratyński; U Kańska; I Petry; Z Szewczuk

doi:10.18388/abp.2001_3879

Evaluation of high temperature glycation of proteins and peptides by electrospray ionization mass spectrometry.

P Stefanowicz Faculty of Chemistry, University of Wrocław, Poland. stp@wchuwr.chem.uni.wroc.pl;
J Boratyński
U Kańska
I Petry
Z Szewczuk

DOI: https://doi.org/10.18388/abp.2001_3879

Abstract

Recently Boratyński & Roy (Glycoconjugate J., 1998, 15, 131) described a fast and convenient procedure for the synthesis of glycoconjugates. In the present study we used ESI-MS and circular dichroism as tools to analyze non-enzymatic glycation products of proteins and peptides. We discuss influence of reaction conditions on the rate of glycation of lysozyme. We analyze for the first time collision induced dissociation spectra of the obtained peptide conjugates.

Published

2001-12-31

Issue

Vol. 48 No. 4 (2001)

Section

Articles

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