Influence of organic solvents on papain kinetics.

M Szabelski; K Stachowiak; W Wiczk

doi:10.18388/abp.2001_3891

Influence of organic solvents on papain kinetics.

M Szabelski Faculty of Chemistry, University of Gdańsk, Poland.;
K Stachowiak
W Wiczk

DOI: https://doi.org/10.18388/abp.2001_3891

Abstract

Papain activity was studied in water-organic solvent mixtures using the fluorogenic substrate Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans. The increase of organic solvent (MeOH, EtOH, iPrOH, TFE, MeCN, (MeO)2Et and DMF) concentration in the mixture caused a substantial decrease the initial rate of papain-catalyzed hydrolysis. Moreover, the number of papain active sites decreased with the increase of DMF and MeOH concentration.

Published

2001-12-31

Issue

Vol. 48 No. 4 (2001)

Section

Articles

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