Electroporated intact BY-2 tobacco culture cells as a model of transient expression study.

E Kościańska; K Wypijewski

doi:10.18388/abp.2001_3900

Electroporated intact BY-2 tobacco culture cells as a model of transient expression study.

E Kościańska Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznań, Poland.;
K Wypijewski

DOI: https://doi.org/10.18388/abp.2001_3900

Abstract

Transfer of foreign genes into plant cells can be accomplished by several methods: agrobacterium-mediated, microinjection, biolistic particle bombardment and electroporation. The last one is frequently used for transfection of plant protoplasts for transient gene expression. Electroporation is a simple procedure and allows transfecting a large number of cells at one time. Square wave-modulated porators are the most efficient for introducing expression cassettes into plant protoplasts. Based on a protocol developed by Wu & Feng (Plant Cell Reports, 1999, 18, 381-386), we optimized conditions for transfection of intact Nicotiana tabacum BY-2 cells using square wave-modulated electroporator. To simplify screening for transfected gene expression we used constructs with a GFP marker gene.

Published

2001-09-30

Issue

Vol. 48 No. 3 (2001)

Section

Articles

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